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Cloning and sequencing of a gene encoding of aldehyde oxidase in Pseudomonas sp. AIU 362

Paper ID Volume ID Publish Year Pages File Format Full-Text
20678 43186 2012 5 PDF Available
Title
Cloning and sequencing of a gene encoding of aldehyde oxidase in Pseudomonas sp. AIU 362
Abstract

We have cloned a gene encoding an aldehyde oxidase (ALOD) oxidized glyoxal but not glyoxylic acid from Pseudomonas sp. AIU 362. The ALOD gene contained an open reading frame consisting of 888 nucleotides corresponding to 295 amino acid residues. The deduced amino acid sequence exhibited a high similarity to those of 3-hydroxyisobutyrate dehydrogenases (3-HIBDHs). We expressed the cloned gene as an active product in Escherichia coli BL21 cells. The productivity (total units per culture broth volume) of the recombinant ALOD expressed in E. coli BL21 was 20,000-fold higher than that of ALOD in Pseudomonas sp. AIU 362. The recombinant ALOD exhibited ALOD activity and 3-HIBDH activity. The 3-HIBDH from Pseudomonas putida KT2440 also exhibited ALOD activity. Thus, the ALOD from Pseudomonas sp. AIU 362 and 3-HIBDH from P. putida KT2440 were classified into the same enzyme group.

Keywords
Aldehyde oxidase; 3-Hydroxyisobutyrate dehydrogenase; Glyoxal; Glyoxylic acid; Pseudomonas
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Cloning and sequencing of a gene encoding of aldehyde oxidase in Pseudomonas sp. AIU 362
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 1, July 2012, Pages 28–32
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us