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In vitro selection of bacteriophage promoters employing a terminally capped template DNA and a streptavidin-binding aptamer

Paper ID Volume ID Publish Year Pages File Format Full-Text
20693 43186 2012 3 PDF Available
Title
In vitro selection of bacteriophage promoters employing a terminally capped template DNA and a streptavidin-binding aptamer
Abstract

In vitro selection system for the isolation of bacteriophage promoters was developed. Employing the system, one round of the selection achieved ∼20–30 fold enrichment of an active promoter from mixtures with an inactive one. In addition, active T7 promoter variants were successfully selected from a library with six randomized bases.

Keywords
T7 RNA polymerase; SP6 RNA polymerase; RNA aptamer; In vitro transcription; Promoter mutationRNAP, RNA polymerase
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In vitro selection of bacteriophage promoters employing a terminally capped template DNA and a streptavidin-binding aptamer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 1, July 2012, Pages 110–112
Authors
,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us