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Isolation and characterization of a mutant recombinant Saccharomyces cerevisiae strain with high efficiency xylose utilization

Paper ID Volume ID Publish Year Pages File Format Full-Text
20705 43187 2013 10 PDF Available
Title
Isolation and characterization of a mutant recombinant Saccharomyces cerevisiae strain with high efficiency xylose utilization
Abstract

A recombinant xylose-utilizing Saccharomyces cerevisiae strain carrying one copy of heterologous XYL1 and XYL2 from Pichia stipitis and endogenous XKS1 under the control of the TDH3 promoter in the chromosomal DNA was constructed from the industrial haploid yeast strain NAM34-4C, which showed thermotolerance and acid tolerance. The recombinant S. cerevisiae strain SCB7 grew in minimal medium containing xylose as the sole carbon source, and its shortest generation time (Gshort) was 5 h. From this strain, four mutants showing rapid growth (Gshort = 2.5 h) in the minimal medium were isolated. The mutants carried four mutations that were classified into three linkage groups. Three mutations were dominant and one mutation was recessive to the wild type allele. The recessive mutation was in the PHO13 gene encoding para-nitrophenyl phosphatase. The other mutant genes were not linked to TAL1 gene encoding transaldolase. When the mutants and their parental strain were used for the batch fermentation in a complex medium at pH 4.0 containing 30 g/L xylose at 35°C with shaking (60 rpm) and an initial cell density (Absorbance at 660 nm) of 1.0, all mutants showed efficient ethanol production and xylose consumption from the early stage of the fermentation culture. In two mutants, within 24 h, 4.8 g/L ethanol was produced, and the ethanol yield was 47%, which was 1.4 times higher than that achieved with the parental strain. The xylose concentration in the medium containing the mutant decreased linearly at a rate of 1 g/L/h until 24 h.

Keywords
Saccharomyces cerevisiae; Bioethanol; High efficiency of xylose utilization; Spontaneous mutant; Recombinant strain
First Page Preview
Isolation and characterization of a mutant recombinant Saccharomyces cerevisiae strain with high efficiency xylose utilization
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 116, Issue 6, December 2013, Pages 706–715
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering