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Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
20710 43187 2013 6 PDF Available
Title
Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering
Abstract

Reconstructed liver has been desired as a liver substitute for transplantation. However, reconstruction of a whole liver has not been achieved because construction of a vascular network at an organ scale is very difficult. We focused on decellularized liver (DC-liver) as an artificial scaffold for the construction of a hierarchical vascular network. In this study, we obtained DC-liver and the tubular network structure in which both portal vein and hepatic vein systems remained intact. Furthermore, endothelialization of the tubular structure in DC-liver was achieved, which prevented blood leakage from the tubular structure. In addition, hepatocytes suspended in a collagen sol were injected from the surroundings using a syringe as a suitable procedure for liver cell inoculation. In summary, we developed a base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering.

Keywords
Decellularized liver; Liver tissue engineering; Vascular-tree network; Hepatocyte; Endothelialization
First Page Preview
Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 116, Issue 6, December 2013, Pages 740–745
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering