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Enhanced withanolide production by overexpression of squalene synthase in Withania somnifera

Paper ID Volume ID Publish Year Pages File Format Full-Text
20731 43188 2013 6 PDF Available
Title
Enhanced withanolide production by overexpression of squalene synthase in Withania somnifera
Abstract

Withania somnifera commonly known as Ashwagandha, is held in high repute in traditional Indian medicine, largely due to the presence of steroidal lactone phytocompounds collectively known as withanolides, such as withanolide A, withaferin A and withanone. These withanolides have diverse pharmacological properties and are prospective high-value drug candidates. To meet the ever-increasing demands of these compounds, plant cell technology offers a viable alternative. In this study, a key enzyme in the isoprenoid biosynthetic pathway, namely squalene synthase, was over-expressed in W. somnifera using Agrobacterium tumefaciens as a transformation vehicle. The cell suspension cultures were developed to assess its effect on withanolide synthesis. The study demonstrated that a significant 4-fold enhancement in squalene synthase activity and 2.5-fold enhancement in withanolide A content were observed in the suspension cultures, as compared to the non-transformed cell cultures. Further, the transformed cell suspension cultures also produced withaferin A, which was absent in the non-transformed cell cultures.

Keywords
Withanolides; Withania; Ashwagandha; Squalene synthase overexpression; Cell culture; Callus
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Enhanced withanolide production by overexpression of squalene synthase in Withania somnifera
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 6, June 2013, Pages 680–685
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us