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Installation of orthogonality to the interface that assembles two modular domains in the Tetrahymena group I ribozyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
20738 43189 2014 6 PDF Available
Title
Installation of orthogonality to the interface that assembles two modular domains in the Tetrahymena group I ribozyme
Abstract

Two modular elements (P5abc and ΔP5) in the Tetrahymena group I ribozyme can be separated physically to generate a two-piece ribozyme derivative consisting of a separately prepared P5abc (P5 RNA) and the rest of the intron (ΔP5 RNA). Molecular recognition in the interface assembling P5 RNA and ΔP5 RNA is strong and specific, and the catalytic ability of the two-piece ribozyme is comparable to that of the parent unimolecular ribozyme. We designed alternative P14 (L5c-L2) interacting modules participating in the assembly of P5 and ΔP5 and investigated their ability in the context of complex formation of the two-piece ribozyme and in vivo splicing of the unimolecular intron ribozyme. Combined use of alternative P14 and L5b-P6 interacting modules provided robust orthogonality to the P5/ΔP5 assembly interface of the bimolecular complex.

Keywords
Ribozyme; RNA; GNRA tetraloop; Tetraloop receptor; Kissing loop
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Installation of orthogonality to the interface that assembles two modular domains in the Tetrahymena group I ribozyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 117, Issue 4, April 2014, Pages 407–412
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us