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Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A

Paper ID Volume ID Publish Year Pages File Format Full-Text
20759 43190 2013 4 PDF Available
Title
Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A
Abstract

Streptococcus pyogenes sortase A (SpSrtA) was transduced into mammalian cells. Then SpSrtA-mediated intracellular circularization of a model protein was confirmed in a time and dose-dependent manner by Western blotting analysis. Direct transduction of SpSrtA is expected to be a strong tool for more conditional specific protein modification in mammalian cells.

Keywords
Sortase A; Protein transduction; Mammalian cell; Protein modification; Cyclization
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Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 116, Issue 3, September 2013, Pages 298–301
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us