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Nanoneedle insertion into the cell nucleus does not induce double-strand breaks in chromosomal DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
20775 43190 2013 6 PDF Available
Title
Nanoneedle insertion into the cell nucleus does not induce double-strand breaks in chromosomal DNA
Abstract

An atomic force microscope probe can be formed into an ultra-sharp cylindrical shape (a nanoneedle) using micro-fabrication techniques such as focused ion beam etching. This nanoneedle can be effectively inserted through the plasma membrane of a living cell to not only access the cytosol, but also to penetrate through the nuclear membrane. This technique shows great potential as a tool for performing intranuclear measurements and manipulations. Repeated insertions of a nanoneedle into a live cell were previously shown not to affect cell viability. However, the effect of nanoneedle insertion on the nucleus and nuclear components is still unknown. DNA is the most crucial component of the nucleus for proper cell function and may be physically damaged by a nanoneedle. To investigate the integrity of DNA following nanoneedle insertion, the occurrence of DNA double-strand breaks (DSBs) was assessed. The results showed that there was no chromosomal DNA damage due to nanoneedle insertion into the nucleus, as indicated by the expression level of γ-H2AX, a molecular marker of DSBs.

Keywords
Nanoneedle; Atomic force microscope; Cell nucleus; γ-H2AX; UV-irradiation; Double-strand breaks
First Page Preview
Nanoneedle insertion into the cell nucleus does not induce double-strand breaks in chromosomal DNA
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 116, Issue 3, September 2013, Pages 391–396
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering