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Cordysobin, a novel alkaline serine protease with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Cordyceps sobolifera

Paper ID Volume ID Publish Year Pages File Format Full-Text
20785 43191 2012 6 PDF Available
Title
Cordysobin, a novel alkaline serine protease with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Cordyceps sobolifera
Abstract

A novel serine protease, designated as cordysobin, was purified from dried fruiting bodies of the mushroom Cordyceps sobolifera. The isolation procedure utilized ion exchange chromatography on DEAE-cellulose and SP-Sepharose followed by gel filtration on Superdex 75. The protease did not adsorb on DEAE-cellulose but bound to SP-Sepharose. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the protease resolved as a single band with an apparent molecular mass of 31 kDa. Its optimal pH was 10.0, and the optimal temperature was 65°C. The protease displayed a Km value of 0.41 μM and 13.44 μM·min−1 using Suc-Leu-Leu-Val-Tyr-MCA as substrate at pH 10.0 and 37°C. Protease activity was enhanced by the Fe2+ ion at low concentration range of 1.25–10 mM and was strongly inhibited by Hg2+ up to 1.25 mM. The protease was strongly inhibited by chymostatin and phenylmethylsulfonyl fluoride (PMSF), suggesting that it is a serine protease. It manifested significant inhibitory activity toward HIV-1 reverse transcriptase (RT) with an IC50 value of 8.2 × 10−3 μM, which is the highest anti-HIV-1 RT activity of reported mushroom proteins.

Keywords
Alkaline serine protease; Fruiting bodies; Mushroom; Cordyceps sobolifera; Purification; HIV-1 reverse transcriptase
First Page Preview
Cordysobin, a novel alkaline serine protease with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Cordyceps sobolifera
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 1, January 2012, Pages 42–47
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering