fulltext.study @t Gmail

Large-scale production of saikosaponins through root culturing of Bupleurum falcatum L. using modified airlift reactors

Paper ID Volume ID Publish Year Pages File Format Full-Text
20796 43191 2012 7 PDF Available
Title
Large-scale production of saikosaponins through root culturing of Bupleurum falcatum L. using modified airlift reactors
Abstract

Modification of internal configuration of a bubble column, airlift and stirred tank reactor (10–200 L) was made for root cultures of Bupleurum falcatum L. Agitation with an impeller covered with partition mesh was ineffective for a 10-L modified reactor, because it caused intensive foaming and subsequent overflow of the culture medium even at a low rotation speed of 50 rpm and a low aeration rate of 0.1 vvm (volume per volume of medium). In contrast, efficient aeration through a ceramic sparger placed at the bottom of a 20-L bubble column reactor yielded approximately 25 g/L of dry roots and 500 mg/L of saikosaponin-a and saikosaponin-d over 42 days. On a 200-L scale, however, the roots became flocculated under the upper perforated plate initially positioned near the middle of the reactor, forming a firm disk of roots and a large empty space between the disk and the medium. Thus, the roots had poor contact with the medium, which severely suppressed their growth. To avoid this flocculation, a bottom perforated plate and draft tube were installed as a partitioning device separating the culturing area (outside the draft tube) from the aeration area (inside the draft tube). The draft tube was made of a stainless steel mesh rather than a solid material, and the tube greatly increased the root yield in the 20-L reactor. This configuration was successfully applied at the 200-L scale, yielding 500–600 mg/L of saikosaponin-a and saikosaponin-d over 56 days.

Keywords
Saikosaponin; Bupleurum falcatum L.; Root culture; Large-scale culture; Airlift reactor
First Page Preview
Large-scale production of saikosaponins through root culturing of Bupleurum falcatum L. using modified airlift reactors
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 1, January 2012, Pages 99–105
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us