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Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase

Paper ID Volume ID Publish Year Pages File Format Full-Text
20803 43192 2014 5 PDF Available
Title
Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase
Abstract

Tyrosinase (EC 1.14.18.1) is the key enzyme of most food enzymatic oxidation. Tyrosinase inhibitors are important in food industry. In the present paper, 2-chlorcinnamic acid and 2,4-dichlorocinnamic acid were synthesized and the inhibitory kinetics on mushroom tyrosinase were investigated. The results showed that both compounds synthesized could inhibit tyrosinase activity. For monophenolase activity, both chlorocinnamic acids could extended the lag time and decrease the steady-state activities, 2-chlorcinnamic acid extended the lag time just by 5%, and 2,4-dichlorcinnamic acid extended the lag time more than by 30.4%. For diphenolase activity, the IC50 values of 2-chlorcinnamic acid and 2,4-dichlorocinnamic acid were determined to be 0.765 mM and 0.295 mM, respectively. The inhibition kinetics showed that 2-chlorcinnamic acid and 2,4-dichlorocinnamic acid displayed a reversible and uncompetitive mechanism. The inhibition constants were determined to be 0.348 mM and 0.159 mM, respectively. The research may supply the basis for designing new tyrosinase inhibitors.

Keywords
Chlorcinnamic acid; Synthesis; Mushroom tyrosinase; Inhibition; KineticsDMSO, dimethyl sulfoxide; l-DOPA, l-3,4-dihydroxyphenylalanine; IC50, the inhibitor concentrations leading to 50% activity lost; KIS, equilibrium constant of the inhibitor combining w
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Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 117, Issue 2, February 2014, Pages 142–146
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us