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Fed-batch system for cultivating genetically engineered yeast that produces lactic acid via the fermentative promoter

Paper ID Volume ID Publish Year Pages File Format Full-Text
20835 43193 2013 3 PDF Available
Title
Fed-batch system for cultivating genetically engineered yeast that produces lactic acid via the fermentative promoter
Abstract

A simple fed-batch system for cultivating genetically engineered yeast generating lactate under the regulation of the PDC1 promoter was established. Traditional strategies that avoid occurrence of Crabtree effect, such as respiratory quotient (RQ) control or ethanol control, are not applicable to the strain because of reduced generation of ethanol and CO2 by-products. In this system, the feed rate increased when the pH was >5.0, and decreased when the pH was <5.0. Using this system, cell yields on sucrose increased by approximately 30% compared to that with the conventional RQ control method, due to the early detection of occurrence of Crabtree effect by pH decrease.

Keywords
Fed-batch culture; Yeast; Crabtree effect; Lactic acid; Bioprocess monitoring; Metabolic engineering
First Page Preview
Fed-batch system for cultivating genetically engineered yeast that produces lactic acid via the fermentative promoter
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 2, February 2013, Pages 193–195
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering