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Preparation of immunogen-reduced and biocompatible extracellular matrices from porcine liver

Paper ID Volume ID Publish Year Pages File Format Full-Text
20838 43193 2013 9 PDF Available
Title
Preparation of immunogen-reduced and biocompatible extracellular matrices from porcine liver
Abstract

Decellularized biologic matrices are plausible biomedical materials for the bioengineering in liver transplantation. However, one of the concerns for safe medical application is the lack of objective assessment of the immunogen within the materials and the in vivo immune responses to the matrices. The purpose of this study was the production of immunogen-reduced and biocompatible matrices from porcine liver. In the present study, 0.1% SDS solution was effective for removing DNA fragments and sequences encoding possible immunogenic and viral antigens within the matrices. The PCR analysis showed that galactose-α-1,3 galactose β-1,4-N-acetylglucosamine (1,3 gal), swine leukocyte antigen (SLA), and porcine endogenous retrovirus (PERV) were completely removed in the matrices. Collagen and glycosaminoglycans (GAGs) were preserved over 63%–71%, respectively, compared to those of native liver. The implanted decellularized tissues showed minimal host responses and naturally degraded within 10 weeks. In this study, we produced immunogen-reduced and biocompatible extracellular matrices from porcine liver. Although future investigations would be required to determine the mechanism of the host reaction, this study could provide useful information of porcine liver-derived biologic matrices for liver researches.

Keywords
Biomaterial; Biomatrix; Extracellular matrices (ECM); Porcine; Liver; ImmunogenECM, extracellular matrices; SDS, sodium dodecyl sulfate; TX, Triton X-100; 1,3 gal, galactose-α-1,3 galactose β-1,4-N-acetylglucosamine; SLA-2, swine leukocyte antigen 2; SLA-
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Preparation of immunogen-reduced and biocompatible extracellular matrices from porcine liver
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 2, February 2013, Pages 207–215
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us