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Ethanol production from d-lactic acid by lactic acid-assimilating Saccharomyces cerevisiae NAM34-4C

Paper ID Volume ID Publish Year Pages File Format Full-Text
20855 43194 2013 6 PDF Available
Title
Ethanol production from d-lactic acid by lactic acid-assimilating Saccharomyces cerevisiae NAM34-4C
Abstract

The lactic acid-assimilating yeast Saccharomyces cerevisiae NAM34-4C grew rapidly in minimal d-lactate medium (pH 3.5) at 35°C, compared with minimal l-lactate medium. A laboratory strain, S. cerevisiae S288C, did not grow in either medium at pH 3.5. Strain NAM34-4C produced remarkably high levels of ethanol in YPDL medium at pH 3.5, but not at pH 5.5, when d-lactate was provided as the carbon source. Optimal cultivation conditions for ethanol production from d-lactate by strain NAM34-4C were as follows: shaking speed, 60 rpm; initial pH, 3.0; cultivation temperature, 35°C; yeast extract, 5 g/L; peptone, 10 g/L; and d-lactate, 30 g/L. Under these conditions, strain NAM34-4C produced 2.7 g/L ethanol, which is 18% of the theoretical maximal yield (0.51 × initial d-lactate concentration).

Keywords
Lactic acid; d-isomer; Ethanol; Saccharomyces cerevisiae; Household waste
First Page Preview
Ethanol production from d-lactic acid by lactic acid-assimilating Saccharomyces cerevisiae NAM34-4C
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 116, Issue 1, July 2013, Pages 85–90
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering