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Inhibitory kinetics of DABT and DABPT as novel tyrosinase inhibitors

Paper ID Volume ID Publish Year Pages File Format Full-Text
20869 43195 2013 4 PDF Available
Title
Inhibitory kinetics of DABT and DABPT as novel tyrosinase inhibitors
Abstract

4-Dimethylaminobenzaldehyde-thiosemicarbazone (DABT) and 4-dimethylaminobenzaldehyde-N-phenyl-thiosemicarbazone (DABPT) were synthesized and established by 1H and 13C NMR and mass spectrum. Both compounds were evaluated for their inhibition activities on mushroom tyrosinase and their anti-tyrosinase kinetics was investigated. The results showed that both compounds exhibited significant inhibitory effects on activity of monophenolase and diphenolase; DABT and DABPT decreased the steady-state rate with 1.54 μM and 1.78 μM as their IC50 values respectively. The inhibitory effects of diphenolase activity exhibited sharp in a dose-dependent manner and their IC50 values were estimated as 2.01 μM and 0.80 μM, respectively. Kinetic analysis showed that their inhibition mechanism was reversible. The inhibition type of DABT was mix-type with inhibition constants KI = 1.77 μM and KIS = 6.49 μM, while that of DABPT displays non-competitive with the inhibition constant KI = 0.77 μM.

Keywords
Mushroom tyrosinase; Benzaldehyde thiosemicarbazone derivatives; Inhibition; Kinetics; SynthesisDABPT, 4-dimethylaminobenzaldehyde-N-phenyl-thiosemicarbazone; DABT, 4-dimethylaminobenzaldehyde-thiosemicarbazone; DMSO, dimethyl sulfoxide; l-DOPA, l-3,4-dih
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Inhibitory kinetics of DABT and DABPT as novel tyrosinase inhibitors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 5, May 2013, Pages 514–517
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us