Acyl migration evaluation in monoacylglycerols from Echium plantagineum seed oil and Marinol
Production of 2-monoacylglycerols (2-MAGs) by selective hydrolysis of the triacylglycerols (TAGs) of Echium plantagineum seed oil and Marinol and further purification was carried out. Three purification methods, including silica gel column chromatography, liquid–liquid extraction and low-temperature crystallization were assayed. Partial acyl migration during the purification step is always observed. Acyl migration rates were similar both for the column chromatography and for the liquid–liquid extraction methods, and resulted in 1-MAG/2-MAG ratios higher than 1.0. Fatty acid (FA) profiles of 2-MAGs after enzyme hydrolysis showed that the major FAs were stearidonic acid (56.9% of total FA in 2-position) and docosahexaenoic acid (63.6% of total FA in 2-position) for E. plantagineum seed oil and Marinol, respectively.
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 5, May 2013, Pages 518–522