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Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses

Paper ID Volume ID Publish Year Pages File Format Full-Text
20872 43195 2013 5 PDF Available
Title
Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses
Abstract

Hyaluronan (HA) synthesis by microalgal Chlorella cells in combination with chloroviruses represents a unique eco-friendly process for converting solar energy and CO2 into useful materials. However, at the final stage of viral infection, infected host cells are completely lysed, and thus HA should be harvested before cell lysis. In the current study, two methods were investigated to improve the yield of HA: (i) adopting slow-growing chlorovirus isolates and (ii) modification of the virus replication process using an inhibitor of DNA synthesis, aphidicolin. Compared with Paramecium bursaria Chlorella virus type 1 (PBCV-1), the prototype chlorovirus, slow-growing virus isolates (CVO1 and CVTS1) produced a 1.5 times higher concentration of HA in infected Chlorella cultures. Furthermore, addition of aphidicolin, an inhibitor of DNA synthesis, delayed virus replication and increased the final concentration of HA 1.5-fold that of cultures without the addition of aphidicolin. Therefore, a 2- to 3-fold increase in the yield of HA by the Chlorella-virus system was attained by using slow-growing viral isolates and the addition of aphidicolin.

Keywords
Hyaluronan; Biosynthesis pathway; Chlorovirus; Infection cycle; AphidicolinGFAT, glutamine:fructose-6-phosphate amidotransferase; GlcA, glucuronic acid; GlcNAc, N-acetylglucosamine; HA, hyaluronan; HAS, hyaluronan synthase; UGDH, UDP-glucose dehydrogenase
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Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 5, May 2013, Pages 527–531
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us