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Novel screening system for protein–protein interactions by bimolecular fluorescence complementation in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
20886 43196 2011 5 PDF Available
Title
Novel screening system for protein–protein interactions by bimolecular fluorescence complementation in Saccharomyces cerevisiae
Abstract

For high-throughput screening of protein–protein interactions, we have developed a novel yeast screening system using Bimolecular fluorescence complementation (BiFC). Two yeast plasmids, in which genes of heterodimerized peptides LZA and LZB were each fused with those of non-fluorescent half fragments of Kusabira-Green mutant (mKG2), were transformed into a- and α-type yeast, respectively. Mating of them gave a library, which was screened by following green fluorescence resulted from LZA-LZB interaction. The method showed potential ability to detect the positive clones from a model library, in which green-fluorescent and non-fluorescent yeast was mixed in a ratio of 1:675.

Keywords
Saccharomyces cerevisiae; Bimolecular fluorescence complementation (BiFC); Protein–protein interactions; Flow cytometry; Fluorescence screening
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Novel screening system for protein–protein interactions by bimolecular fluorescence complementation in Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 4, April 2011, Pages 397–401
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us