fulltext.study @t Gmail

Purification and characterization of an extracellular lipase from Mucor hiemalis f. corticola isolated from soil

Paper ID Volume ID Publish Year Pages File Format Full-Text
20908 43197 2012 6 PDF Available
Title
Purification and characterization of an extracellular lipase from Mucor hiemalis f. corticola isolated from soil
Abstract

We have screened 39 microfungi isolates originated from soil in terms of lipolytic activity. Out of all screened, a novel strain of Mucor hiemalis f. corticola was determined to have the highest lipase activity. The extracellular lipase was produced in response to 2% glucose and 2.1% peptone. The lipase was purified 12.63-folds with a final yield of 27.7% through following purification steps; ammonium sulfate precipitation, dialysis, gel filtration column chromatography and ion exchange chromatography, respectively. MALDI-TOF MS analysis revealed 31% amino-acid identity to a known lipase from Rhizomucor miehei species. The molecular weight of the lipase was determined as 46 kDa using SDS-PAGE and analytical gel filtration. Optimal pH and temperature of the lipase were determined as 7.0 and 40°C, respectively. The enzyme activity was observed to be stable at the pH range of 7.0–9.0. Thermostability assays demonstrated that the lipase was stable up to 50°C for 60 min. The lipase was more stable in ethanol and methanol than other organic solvents tested. Furthermore, the activity of the lipase was slightly enhanced by SDS and PMSF. In the presence of p-NPP as substrate, Km and Vmax values of the lipase were calculated by Hanes–Woolf plot as 1.327 mM and 91.11 μmol/min, respectively.

Keywords
Soil isolate; Screening; Mucor hiemalis f. corticola; Extracellular lipase; Purification; Characterization
First Page Preview
Purification and characterization of an extracellular lipase from Mucor hiemalis f. corticola isolated from soil
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 4, October 2012, Pages 385–390
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us