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Covalent immobilization of ascorbate oxidase onto polycarbonate strip for l-ascorbic acid detection

Paper ID Volume ID Publish Year Pages File Format Full-Text
20911 43197 2012 3 PDF Available
Title
Covalent immobilization of ascorbate oxidase onto polycarbonate strip for l-ascorbic acid detection
Abstract

Herein, a simple and rapid method is described for detection of l-ascorbic acid by ascorbate oxidase immobilized onto polycarbonate strip pre-activated by 1-fluoro-2-nitro-4-azidobenzene in photochemical reaction. Covalent attachment of ascorbate oxidase was confirmed by XPS studies. The immobilized-ascorbate oxidase shows higher pH, thermal and storage stability in comparison to free enzyme.

Keywords
Polycarbonate; 1-Fluoro-2-nitro-4-azidobenzene; Activated surface; Covalent immobilization; Ascorbate oxidase; Ascorbic acid
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Covalent immobilization of ascorbate oxidase onto polycarbonate strip for l-ascorbic acid detection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 4, October 2012, Pages 402–404
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us