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Molecular identification of marine yeast and its spectroscopic analysis establishes unsaturated fatty acid accumulation

Paper ID Volume ID Publish Year Pages File Format Full-Text
20913 43197 2012 7 PDF Available
Title
Molecular identification of marine yeast and its spectroscopic analysis establishes unsaturated fatty acid accumulation
Abstract

Marine microbes are competent organisms, some of which can accumulate large amounts of lipids. A yeast strain, Rhodotorula mucilaginosa AMCQ8A was isolated from the marine water of the Queenscliff region, Victoria, Australia. The yeast isolate was identified by sequencing 18s rDNA genes. Scanning electron microscopy images revealed scars on the surface of the yeast cells. The Fourier transform infrared spectroscopy microspectroscopy studies demonstrated the presence of unsaturated fatty acids by differential microscopic analysis. The sharp band at 1745 cm−1 was represented by ν(CO) stretches of ester functional groups from lipids and fats, and therefore indicated the presence of total lipids produced by the cells. Over 65% of the fatty acids from the yeast strain were analyzed as C16 and C18:1 with omega-3 content from about 6% to 7%. Thus, this marine-derived yeast could be a potential source of lipids, including omega-3 fatty acids.

Keywords
Marine; Oleaginous; Unsaturated fatty acids; Omega-3; Fourier transform infrared spectroscopy (FTIR)
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Molecular identification of marine yeast and its spectroscopic analysis establishes unsaturated fatty acid accumulation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 4, October 2012, Pages 411–417
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us