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Improvement of the growth defect in salt- and ethanol-tolerant yeast mutagenized with error-prone DNA polymerization by using backcross cell fusion

Paper ID Volume ID Publish Year Pages File Format Full-Text
20925 43197 2012 3 PDF Available
Title
Improvement of the growth defect in salt- and ethanol-tolerant yeast mutagenized with error-prone DNA polymerization by using backcross cell fusion
Abstract

Salt- and ethanol-tolerant mutants of Saccharomyces cerevisiae, isolated from the uracil-requiring mutant derived from Taiken No. 396 by proofreading-deficient DNA polymerization, showed less growth than their parent strain. The fusants, between these tolerant mutants and the lysine-requiring mutant from Taiken No. 396 obtained by the protoplast fusion, indicated improved growth.

Keywords
Salt-tolerant yeast; Ethanol-tolerant yeast; Cell fusion; Backcross; Error-prone DNA polymerase; Saccharomyces cerevisiae
First Page Preview
Improvement of the growth defect in salt- and ethanol-tolerant yeast mutagenized with error-prone DNA polymerization by using backcross cell fusion
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 4, October 2012, Pages 476–478
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering