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Ethanol fermentation driven by elevated expression of the G1 cyclin gene CLN3 in sake yeast

Paper ID Volume ID Publish Year Pages File Format Full-Text
20936 43198 2011 6 PDF Available
Title
Ethanol fermentation driven by elevated expression of the G1 cyclin gene CLN3 in sake yeast
Abstract

Cellular and subcellular morphology reflects the physiological state of a cell. To determine the physiological nature of sake yeast with superior fermentation properties, we quantitatively analyzed the morphology of sake yeast cells by using the CalMorph system. All the sake strains examined here exhibited common morphological traits that are typically observed in the well-characterized whiskey (whi) mutants that show accelerated G1/S transition. In agreement with this finding, the sake strain showed less efficient G0/G1 arrest and elevated expression of the G1 cyclin gene CLN3 throughout the fermentation period. Furthermore, deletion of CLN3 remarkably impaired the fermentation rate in both sake and laboratory strains. Disruption of the SWI6 gene, a transcriptional coactivator responsible for Cln3p-mediated G1/S transition, also resulted in a decreased fermentation rate, whereas whi mutants exhibited significant improvement in the fermentation rate, demonstrating positive roles of Cln3p and its downstream signalling pathway in facilitating ethanol fermentation. The combined results indicate that enhanced induction of CLN3 contributes to the high fermentation rate of sake yeast, which are natural whi mutants.

Keywords
Sake yeast; Saccharomyces cerevisiae; Ethanol fermentation; CalMorph system; G1 cyclin; CLN3; whi mutants; G1/S transition
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Ethanol fermentation driven by elevated expression of the G1 cyclin gene CLN3 in sake yeast
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 6, December 2011, Pages 577–582
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us