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Effects of flow field on the metabolic characteristics of Streptomyces lincolnensis in the industrial fermentation of lincomycin

Paper ID Volume ID Publish Year Pages File Format Full-Text
20954 43199 2013 5 PDF Available
Title
Effects of flow field on the metabolic characteristics of Streptomyces lincolnensis in the industrial fermentation of lincomycin
Abstract

In this work, the flow field in the existing fermentor with radial-flow impellers (C1) was studied using the computational fluid dynamics (CFD) software package Fluent, then the fermentor with radial-axial flow impellers (C2) was constructed and was compared with the C1 fermentor by CFD and experimental research. The simulation results revealed that the flow field in C2 fermentor had characteristics such as higher turbulent kinetic energy, gas holdup and shear rates. Metabolic variables of Streptomyces lincolnensis in the two fermentors such as carbon and nitrogen source consumption rates, specific growth rates (μ), hyphae morphologies, and lincomycin productivities were further explored. The correlation analysis between the experimental measurements and the simulation results indicated that the hyphae clustering and dry cell weight (DCW) decreasing at production stage were eliminated in C2 fermentor, which had higher gas volumetric mass transfer coefficient (KLa), dissolved oxygen (DO) concentration and consumption rates of nutrient materials. When C2 was employed in the fermentor, the specific growth rate of S. lincolnensis at growth stage was higher, and the maintenance metabolism together with secondary metabolism at production stage was kept at higher levels. As a result, the yield of lincomycin was achieved 7039 μg ml−1 when the 60 m3 industrial fermentor was equipped with C2, which was increased by 46% compared to that obtained in the C1 fermentor.

Keywords
Streptomyces lincolnensis; Lincomycin fermentation; Metabolic characteristic; Computational fluid dynamics; Simulation of flow field
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Effects of flow field on the metabolic characteristics of Streptomyces lincolnensis in the industrial fermentation of lincomycin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 1, January 2013, Pages 27–31
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us