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Role of the glyoxylate pathway in acetic acid production by Acetobacter aceti

Paper ID Volume ID Publish Year Pages File Format Full-Text
20955 43199 2013 5 PDF Available
Title
Role of the glyoxylate pathway in acetic acid production by Acetobacter aceti
Abstract

Wild-type Acetobacter aceti NBRC 14818 possesses genes encoding isocitrate lyase (aceA) and malate synthase (glcB), which constitute the glyoxylate pathway. In contrast, several acetic acid bacteria that are utilized for vinegar production lack these genes. Here, an aceA–glcB knockout mutant of NBRC 14818 was constructed and used for investigating the role of the glyoxylate pathway in acetate productivity. In medium containing ethanol as a carbon source, the mutant grew normally during ethanol oxidation to acetate, but exhibited slower growth than that of the wild-type strain as the accumulated acetate was oxidized. The mutant grew similarly to that of the wild-type strain in medium containing glucose as a carbon source, indicating that the glyoxylate pathway was not necessary for glucose utilization. However, in medium containing both ethanol and glucose, the mutant exhibited significantly poorer growth and lower glucose consumption compared to the wild-type strain. Notably, the mutant oxidized ethanol nearly stoichiometrically to acetate, which was retained in the medium for a longer period of time than the acetate produced by wild-type strain. The features of the aceA–glcB knockout mutant revealed here indicate that the lack of the glyoxylate pathway is advantageous for industrial vinegar production by A. aceti.

Keywords
Glyoxylate pathway; Acetate production; Ethanol; Incomplete oxidation; Knockout mutant; Acetobacter acetiADH, alcohol dehydrogenase; ALDH, aldehyde dehydrogenase; CFE, cell-free extract; DCIP, 2,6-dichlorophenolindophenol; PMS, phenazine methosulfate; PQQ
First Page Preview
Role of the glyoxylate pathway in acetic acid production by Acetobacter aceti
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 1, January 2013, Pages 32–36
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering