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Preparation of a γ-glutamylcysteine-enriched yeast extract from a newly developed GSH2-deficient strain

Paper ID Volume ID Publish Year Pages File Format Full-Text
20958 43199 2013 5 PDF Available
Title
Preparation of a γ-glutamylcysteine-enriched yeast extract from a newly developed GSH2-deficient strain
Abstract

Gamma-glutamylcysteine (γ-GC), the precursor of glutathione (GSH), may have significant health benefits as a dietary supplement, but there are few cost-effective methods available for its large-scale production. We developed an efficient method for producing γ-GC in a mutant yeast strain using a three-step breeding procedure and a unique cultivation process. In the first breeding step, we prepared a glutathione synthetase (GSH2  )-deficient yeast mutant. In the second step, selenate (SeO42−SeO42−) sensitivity was introduced by crossing the GSH2-deficient mutant with a strain harboring the met30 mutation. In the final step, pantothenic acid auxotrophy was introduced by ethyl methanesulfonate mutagenesis. The isolated strain displayed significantly enhanced cellular γ-GC when cultivated in synthetic medium without pantothenic acid, reaching a maximum level of 4.39% of dry cell weight. Using this strain, we were able to prepare a yeast extract containing approximately 13% γ-GC (w/w), which is markedly higher than the reported value (0.3%) of commercially available yeast extracts. The present method may facilitate large-scale γ-GC production for investigating the nutritive value and other benefits of dietary γ-GC.

Keywords
Gamma-glutamylcysteine; Pantothenic acid auxotrophy induction; Functional foodstuff; Yeast extract; Glutathione-deficient yeast mutant
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Preparation of a γ-glutamylcysteine-enriched yeast extract from a newly developed GSH2-deficient strain
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 1, January 2013, Pages 50–54
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us