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Development of a microfabricated device for low-voltage electropermeabilization of adherent cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
20984 43200 2013 6 PDF Available
Title
Development of a microfabricated device for low-voltage electropermeabilization of adherent cells
Abstract

A microfabricated device for low-voltage electropermeabilization of adherent cells has been developed. The device, which is fabricated on a cover glass using mainly transparent materials, allows optical observation of cells through it. This device consists of an array of 6-μm circular electrodes connected via a transparent and conductive layer made of ITO (indium tin oxide) to give pulsed voltages for electropermeabilization. The surface of the electrode is coated with a thin Au film to reduce impedance. The insulation layer contains holes aligned on the electrodes to constrict the electric field so that the applied voltage drops mainly at the hole and is effectively used for electropermeabilization. Adherent cells for electropermeabilization are cultured on the device and those in the vicinity of the holes are influenced by the relatively strong electric field induced by the holes in the insulation layer. We demonstrated the culture of adherent cells on the device and their optical observation by phase-contrast microscopy. We further demonstrated the delivery of YO-PRO-1 into various cells including HeLa, NIH3T3, and smooth muscle cells, by applying pulsed voltages of 6.0 V.

Keywords
Electropermeabilization; Low-voltage; Microfabrication; Photoresist; HeLa
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Development of a microfabricated device for low-voltage electropermeabilization of adherent cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 115, Issue 3, March 2013, Pages 314–319
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us