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Development of macrolide lactone antibiotic brefeldin A fermentation process with Eupenicillium brefeldianum ZJB082702

Paper ID Volume ID Publish Year Pages File Format Full-Text
20992 43201 2012 6 PDF Available
Title
Development of macrolide lactone antibiotic brefeldin A fermentation process with Eupenicillium brefeldianum ZJB082702
Abstract

In this work, a robust brefeldin A-synthesizing fungus, Eupenicillium brefeldianum ZJB082702, was bred from a Murraya paniculata endophytic fungus E. brefeldianum A1163. Using one-factor-at-a-time experimental design, optimization of media composition for E. brefeldianum ZJB082702 fermenting brefeldin A was conducted. Outcomes indicated that mixed carbon source and mixed nitrogen source were of c ritical importance to brefeldin A fermentation. After 6 d culture in the optimized fermentation media, composed of (g l−1) 13.33 starch, 26.67 glucose, 1.0 yeast extract powder, 1.0 corn steep liquor, 0.5 soybean meal, 0.75 NaNO3, 2.5 malt extract, 6.0 CaCO3, 3.0 MgSO4, 4.0 KH2PO4, 1.0 × 10−2 CuSO4, brefeldin A yield peaked at 1304.7 mg l−1, 648.2 mg l−1 in 500 ml baffled flask and 15 l stirred fermentor respectively, formed as a growth associated type of secondary metabolite based on fermentation profile analysis.

► A brefeldin A overproducing fungus, E. brefeldianum ZJB082702, was bred. ► Mixed carbon, nitrogen sources were critical to brefeldin A synthesis. ► Brefeldin A is synthesized by E. brefeldianum in a growth associated manner. ► Six-day culture in baffled flasks gave a peak brefeldin A yield of 1304.7 mg l−1. ► Brefeldin A titer peaked at 648.2 mg l−1 in 15 l stirred fermentor.

Keywords
Brefeldin A; Identification; Eupenicillium brefeldianum; Breeding; Fermentation
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Development of macrolide lactone antibiotic brefeldin A fermentation process with Eupenicillium brefeldianum ZJB082702
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 3, September 2012, Pages 262–267
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us