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Characteristics of the high malic acid production mechanism in Saccharomyces cerevisiae sake yeast strain No. 28

Paper ID Volume ID Publish Year Pages File Format Full-Text
20995 43201 2012 5 PDF Available
Title
Characteristics of the high malic acid production mechanism in Saccharomyces cerevisiae sake yeast strain No. 28
Abstract

We characterized a high malic acid production mechanism in sake yeast strain No. 28. No considerable differences in the activity of the enzymes that were involved in malic acid synthesis were observed between strain No. 28 and its parent strain, K1001. However, compared with strain K1001, which actively took up rhodamine 123 during staining, the cells of strain No. 28 were only lightly stained, even when cultured in high glucose concentrations. In addition, malic acid production by the respiratory-deficient strain of K1001 was 2.5-fold higher than that of the wild-type K1001 and wild-type No. 28. The findings of this study demonstrated that the high malic acid production by strain No. 28 is attributed to the suppression of mitochondrial activity.

Keywords
Saccharomyces cerevisiae; Sake yeast; Malic acid; Mitochondria; Glucose repression
First Page Preview
Characteristics of the high malic acid production mechanism in Saccharomyces cerevisiae sake yeast strain No. 28
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 3, September 2012, Pages 281–285
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering