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Flow cytometric analysis of lectin–oligosaccharide interactions by using fluorescent oligosaccharide probes

Paper ID Volume ID Publish Year Pages File Format Full-Text
21026 43202 2011 3 PDF Available
Title
Flow cytometric analysis of lectin–oligosaccharide interactions by using fluorescent oligosaccharide probes
Abstract

Tetraphenylethylene (TPE) derivatives have strong fluorescence in aggregated state. We report here an oligosaccharide binding assay system using tetraphenylethylene derivatives bearing oligosaccharides with aggregation-induced emission (AIE) characteristics. A tetraphenylethylene derivative bearing 6′-sialyllactose (6′SL) bound to microbeads coated with SSA lectin more effectively than RCA120 lectin. Microbeads that bound to fluorescent oligosaccharide probes could be detected by flow cytometric analysis. Tetraphenylethylene derivatives bearing oligosaccharides are useful for flow cytometric analysis of lectin–oligosaccharide interactions.

Keywords
Oligosaccharide; Oligosaccharide probe; Fluorescent; Flow cytometer; Lectin
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Flow cytometric analysis of lectin–oligosaccharide interactions by using fluorescent oligosaccharide probes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 2, August 2011, Pages 202–204
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us