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Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
21028 43203 2012 5 PDF Available
Title
Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells
Abstract

Susceptibility of human pluripotent stem cells (hPSCs), such as human iPS and embryonic stem (ES) cells, to single-cell dissociation has been a large obstacle to develop the efficient manipulation techniques required for stem cell research. When hPSCs are completely dissociated into single cells, programmed cell death (apoptosis) is immediately induced. A specific inhibitor of Rho-associated protein kinase (ROCK inhibitor), Y-27632, is of particular interest as a useful reagent that allows hPSCs to escape the dissociation-induced apoptosis. ROCK inhibitor has been used in a variety of applications associated with cell dissociation in the process of stem cell research, such as passaging, expansion, cryopreservation, gene transfer, differentiation induction, and cell sorting, suggesting that it may be a crucial reagent for the handling of hPSCs. This article reviews the current applications of ROCK inhibitors to stem cell research from the viewpoint of quality control of hPSCs.

Keywords
Y-27632; ROCK inhibitor; Embryonic stem cells; Dissociation; Adhesion
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Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 6, December 2012, Pages 577–581
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us