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Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of ade1 or ade2 mutants

Paper ID Volume ID Publish Year Pages File Format Full-Text
21035 43203 2012 4 PDF Available
Title
Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of ade1 or ade2 mutants
Abstract

We identified Saccharomyces cerevisiae mutants with 100% higher intracellular glutathione using 1-methyl-3-nitro-1-nitrosoguanidine mutagenesis. This method employs visual selection of the most pigmented colonies among met30 strains carrying ade1 and ade2 mutations. Since the method does not involve genetic engineering, the mutants are suitable for use in the food industry.

Keywords
Visual selection of colonies; Glutathione; Yeast with red pigmentation; Saccharomyces cerevisiae; ade1 mutation; ade2 mutation
First Page Preview
Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of ade1 or ade2 mutants
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 6, December 2012, Pages 615–618
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering