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Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of colonies using the met30 strain

Paper ID Volume ID Publish Year Pages File Format Full-Text
21036 43203 2012 3 PDF Available
Title
Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of colonies using the met30 strain
Abstract

Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium with a high methionine content.

Keywords
Visual selection of colonies; Glutathione; Yeast with red pigmentation; Saccharomyces cerevisiae; ade1 mutation; ade2 mutation
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Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of colonies using the met30 strain
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 6, December 2012, Pages 619–621
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us