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Motility behavior of rpoS-deficient Escherichia coli analyzed by individual cell tracking

Paper ID Volume ID Publish Year Pages File Format Full-Text
21042 43203 2012 5 PDF Available
Title
Motility behavior of rpoS-deficient Escherichia coli analyzed by individual cell tracking
Abstract

Motility is one of the most extensively studied cellular events conducted by bacteria, including Escherichia coli. A motility agar plate assay showed that deletion of the rpoS gene enhanced the apparent motility of the E. coli BW25113 strain, which inherently had negligible motility compared to wild-type E. coli strains, such as MG1655, with no effect on cell growth. This enhancement of motility was accompanied by drastic up-regulation of genes involved in the formation and rotation of flagella. Furthermore, an individual cell motility assay showed that the population of ΔrpoS cells had bimodal motility character, and that a minority of this population exhibited a much higher motility rate. These results support a view that a minority population contributes to increasing in apparent motility of the whole population of ΔrpoS cells.

Keywords
Escherichia coli; Motility; Individual cells; rpoS gene; Flagellum
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Motility behavior of rpoS-deficient Escherichia coli analyzed by individual cell tracking
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 6, December 2012, Pages 652–656
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
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