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A simple fed-batch method for transcription and insect cell-free translation

Paper ID Volume ID Publish Year Pages File Format Full-Text
21046 43203 2012 3 PDF Available
Title
A simple fed-batch method for transcription and insect cell-free translation
Abstract

We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74–112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.

Keywords
Insect cell-free system; Undigested plasmids; One batch reaction; Eukaryotic proteins; High-throughput
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A simple fed-batch method for transcription and insect cell-free translation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 6, December 2012, Pages 677–679
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us