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Resolvase-like serine recombinase mediates integration/excision in the bacteriophage ϕRSM

Paper ID Volume ID Publish Year Pages File Format Full-Text
21047 43204 2011 8 PDF Available
Title
Resolvase-like serine recombinase mediates integration/excision in the bacteriophage ϕRSM
Abstract

ϕRSM1, a filamentous phage infecting Ralstonia solanacearum, encodes an open reading frame (ORF14) that exhibits significant homology to members of the resolvase/invertase subfamily of site-specific serine recombinases. Similar prophages are found in the genomes of various strains of R. solanacearum, R. pickettii, and Burkholderia pseudomallei. We have characterized the integrative and excisive recombination reactions mediated by the ϕRSM1 integrase using in vivo assays. An E. coli plasmid containing the RSM1 orf14 and attP sequences (pT-orf14-attP) was shown to integrate into the attB sequence of R. solanacearum cells. Intermolecular recombination between pT-orf14-attP and pS-attB (a R. solanacearum plasmid containing attB) also occurred in R. solanacearum cells. The products of attP/attB recombination, i.e., attL and attR, were exactly identical to the sequences found in the prophage ϕRSM in R. solanacearum strains. Intramolecular recombination between the attL and attR sequences (excisive recombination) on the pRecomb plasmid was also shown to occur in an orf14-dependent manner. This is the first evidence that a small serine recombinase from the resolvase/invertase group may function in integrative and excisive recombination for filamentous phages.

Keywords
attP–attB; Filamentous phage; Integration–excision; Serine recombinase; Phytopathogen
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Resolvase-like serine recombinase mediates integration/excision in the bacteriophage ϕRSM
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 2, February 2011, Pages 109–116
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us