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Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter

Paper ID Volume ID Publish Year Pages File Format Full-Text
21048 43204 2011 4 PDF Available
Title
Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter
Abstract

Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation.

Keywords
Lentinula edodes; Novel vector; Transformation; Chitin synthase; Gene expression
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Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 2, February 2011, Pages 117–120
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us