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Family M42 aminopeptidase from the syntrophic bacterium Symbiobacterium thermophilum: Characterization using recombinant protein

Paper ID Volume ID Publish Year Pages File Format Full-Text
21051 43204 2011 6 PDF Available
Title
Family M42 aminopeptidase from the syntrophic bacterium Symbiobacterium thermophilum: Characterization using recombinant protein
Abstract

The chromosomal DNA of the syntrophic thermophile Symbiobacterium thermophilum contains open reading frames of the genes encoding family M42 aminopeptidases, Pep1079, Pep1080, and Pep1081. To characterize these peptidases, the genes were cloned into Escherichia coli and overexpressed. Our experiments using the recombinant proteins confirmed that Pep1079, Pep1080, and Pep1081 are components of arginyl or lysinyl aminopeptidases that require Co2+ for enzymatic activity. Coexistence of Pep1079 and Pep1080 is necessary for expressing high peptidase activity. Pep1081 enhances the activity of Pep1079 and Pep1080.

Keywords
Symbiobacterium thermophilum; Family M42 aminopeptidase; Gene cloning; Recombinant protein; Enzyme characterization
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Family M42 aminopeptidase from the syntrophic bacterium Symbiobacterium thermophilum: Characterization using recombinant protein
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 2, February 2011, Pages 134–139
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us