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Biofilm formation by Streptococcus pyogenes: Modulation of exopolysaccharide by fluoroquinolone derivatives

Paper ID Volume ID Publish Year Pages File Format Full-Text
21076 43205 2011 6 PDF Available
Title
Biofilm formation by Streptococcus pyogenes: Modulation of exopolysaccharide by fluoroquinolone derivatives
Abstract

Biofilm formation by Streptococcus pyogenes has been demonstrated as a potentially important mechanism contributing to antibiotic treatment failure. S. pyogenes is the frequent cause of purulent infections in humans and also, it could play a significant role in recurrent and chronic infections. S. pyogenes biofilm communities tend to exhibit significant tolerance to antimicrobial challenge during infections. The fluoroquinolone derivatives have been previously reported from our laboratory as effective agents against human bacterial pathogens. Therefore, in the present study, we observed the effect of these fluoroquinolones on biofilm formation. Quantitative analysis using 2,3-bis (2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide (XTT) at the biofilm inhibitory concentrations (BIC), the compounds 6a, 6c, 7b and 7c reduced 61–71% biofilm and sub-BIC (0.5 and 0.25 BIC) significantly reduced biofilm formation by up to 30–38% and 16–18%, respectively. The Fourier Transform Infrared (FTIR) spectrum of the control and treated S. pyogenes revealed the shift in the chemical entity corresponding to the exopolysaccharide (EPS). The GC/MS analysis showed that the EPS of S. pyogenes has the most abundant neutral sugars l-glucose and d-mannose which is not detected in the fluoroquinolone treated EPS.

Keywords
Streptococcus pyogenes; Fluoroquinolones; Biofilm formation; Exopolysaccharide; Biofilm inhibitory concentration; Confocal laser scanning electron microscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 4, October 2011, Pages 345–350
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us