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Optimization of process parameters for the production of an OTA-hydrolyzing enzyme from Aspergillus niger under solid-state fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
21077 43205 2011 5 PDF Available
Title
Optimization of process parameters for the production of an OTA-hydrolyzing enzyme from Aspergillus niger under solid-state fermentation
Abstract

Ochratoxin A (OTA) is a mycotoxin found in several food and feed products. Due to its acute toxicity, innovative biological strategies to degrade OTA have been sought. In previous studies, Aspergillus niger MUM 03.58 was found to produce one promising OTA-hydrolyzing enzyme for food and feed applications. In this paper, we describe the optimization of a solid-state fermentation (SSF) process to produce this enzyme using the one-factor-at-a-time and the Taguchi experimental design approaches. A preliminary evaluation of the fermentation time, substrate moisture content and type of carbon source was done by the one-factor-at-a-time method. A final maximization of the OTA-hydrolyzing enzyme production was done using Taguchi’s experimental design. An L9 orthogonal array was used to evaluate the effect of four factors at three levels. The substrate composition, the fermentation time, the operating temperature and the moisture content of the substrate were the factors evaluated. The results were tested by ANOVA and optimum conditions for a verification test were determined by statistical calculations. The optimized conditions were 30 g of wheat bran at 70% moisture incubated for 14 days at 25°C. A final productivity of 154 U/g substrate was achieved, which represents an approximately 3.7-fold increase in enzyme yield when compared with the starting point conditions.

Keywords
Aspergillus niger; Ochratoxin A; OTA-hydrolyzing enzyme; Taguchi’s experimental design; Solid-state fermentation
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Optimization of process parameters for the production of an OTA-hydrolyzing enzyme from Aspergillus niger under solid-state fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 4, October 2011, Pages 351–355
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us