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Capillary-composited microfluidic device for heat shock transformation of Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
21082 43205 2011 6 PDF Available
Title
Capillary-composited microfluidic device for heat shock transformation of Escherichia coli
Abstract

This work describes chemical heat shock transformation of foreign plasmid DNA into bacterial host Escherichia coli cells using a capillary-composited microfluidic device. Transformation processes of the loading, mixing, heat shock and recovery of the transformation mixture were carried out automatically in a linear fashion. In addition, by utilizing the capillary with a hollow cylindrical chamber as heating source, simple, low cost local heat shock with accurate heat shock time to transformation mixture was obtained on the microdevice. Results demonstrated that plasmid DNA could be effectively transformed into E. coli, and the transformation efficiency and frequency were as the same level or better than conventional tube-based method. This work complements other microfluidic technologies for potential gene cloning and functional genomics studies.

Keywords
Microfluidic; Capillary; Escherichia coli; Heat shock; Transformation
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Capillary-composited microfluidic device for heat shock transformation of Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 4, October 2011, Pages 373–378
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us