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Comprehension of terminal differentiation and dedifferentiation of chondrocytes during passage cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
21085 43205 2011 7 PDF Available
Title
Comprehension of terminal differentiation and dedifferentiation of chondrocytes during passage cultures
Abstract

A high density collagen type I coated substrate (CL substrate) was used to evaluate the chondrocyte phenotypes in passaged cultures. With increasing age of cell population (population doubling (PD) = 0–14.5), the frequency of non-dividing spindle shaped cells without ALP activity increased, accompanied with an increase in gene expression of collagen type I, meaning the senescence of dedifferentiated cells. At the middle age of cell population (PD = 5.1 and 6.6), the high frequency of polygonal shaped cells with ALP activity existed on the CL substrate together with up-regulated expressions of collagen types II and X, indicating the terminal differentiation of chondrocytes. When the chondrocytes passaged up to the middle age were embedded in collagen gel, the high frequency of single hypertrophic cells with collagen type II formation was recognized, which supports the thought that the high gene expression of collagen type II was attributed to terminal differentiation rather than redifferentiation. These results show that the CL substrate can draw out the potential of terminal differentiation in chondrocytes, which is unattainable by a polystyrene surface, and that the CL substrate can be a tool to evaluate cell quality in three-dimensional culture with the collagen gel.

Keywords
Tissue engineering; Rabbit chondrocytes; Terminal differentiation; Passage cultures; Collagen-coated substrate; Cultured cartilage
First Page Preview
Comprehension of terminal differentiation and dedifferentiation of chondrocytes during passage cultures
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 4, October 2011, Pages 395–401
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering