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Functional replacement of yeast flavocytochrome b2 with bacterial l-lactate dehydrogenase

Paper ID Volume ID Publish Year Pages File Format Full-Text
21088 43206 2010 4 PDF Available
Title
Functional replacement of yeast flavocytochrome b2 with bacterial l-lactate dehydrogenase
Abstract

Using yeast genetic complementation, we show here that Rhodobacter sphaeroidesl-lactate dehydrogenase can functionally replace flavocytochrome b2 in Saccharomyces cerevisiae, only when a matrix-targeting signal of flavocytochrome b2 is fused with the bacterial enzyme. The recombinant l-lactate dehydrogenase may add alternative route of mitochondrial electron transport other than flavocytochrome b2.

Keywords
l-Lactate dehydrogenase; Chimera; Genetic complementation; Matrix targeting; Mitochondrial electron transport
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Functional replacement of yeast flavocytochrome b2 with bacterial l-lactate dehydrogenase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 3, September 2010, Pages 269–272
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us