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Low-cost fermentation medium for alkaline protease production by Bacillus mojavensis A21 using hulled grain of wheat and sardinella peptone

Paper ID Volume ID Publish Year Pages File Format Full-Text
21092 43206 2010 7 PDF Available
Title
Low-cost fermentation medium for alkaline protease production by Bacillus mojavensis A21 using hulled grain of wheat and sardinella peptone
Abstract

Media composition and culture conditions for surfactant stable alkaline protease production by Bacillus mojavensis A21 were optimized using two statistical methods. Plackett–Burman design was applied to find the optimal ingredients and conditions to improve yields. Response surface methodology (RSM), including central composite design, was used to determine the optimal concentrations and conditions. The results indicated that several components, including hulled grain of wheat (HGW), sardinella peptone (SP), NaCl, CaCl2, MgSO4, K2HPO4, KH2PO4, agitation, culture temperature and initial medium pH, had significant effects on production. The statistical model was constructed via central composite design (CCD) using four selected variables (HGW, NaCl, KH2PO4 and K2HPO4). Under the proposed optimized conditions, the protease experimental yield (1860.63 U/mL) closely matched the yield predicted by the statistical model (1838.60 U/mL) with R2 = 0.98. An overall 14.0-fold increase in protease production was achieved using the optimized medium (HGW 30.0 g/L, SP 1.0 g/L, NaCl 2.0 g/L, KH2PO4 1.0 g/L, K2HPO4 0.3 g/L, CaCl2 2.0 g/L, MgSO4 1.0 g/L and pH 9.0, compared with the unoptimized basal medium (starch 10.0 g/L, yeast extract 2.0 g/L, KH2PO4 0.1 g/L, K2HPO4 0.1 g/L, CaCl2 0.5 g/L and pH 8.0; 137 U/mL). A successful and significant improvement (14-fold) in the production of protease by the A21 strain was accomplished using cheap carbon and nitrogen substrates (HGW and SP), which may result in a significant reduction in the cost of medium constituents.

Keywords
Media optimization; Bacillus mojavensis A21; Alkaline detergent proteases; Low-cost media
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Low-cost fermentation medium for alkaline protease production by Bacillus mojavensis A21 using hulled grain of wheat and sardinella peptone
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 3, September 2010, Pages 288–294
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us