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Control of specific growth rate to enhance the production of a novel disintegrin, saxatilin, in recombinant Pichia pastoris

Paper ID Volume ID Publish Year Pages File Format Full-Text
21097 43206 2010 6 PDF Available
Title
Control of specific growth rate to enhance the production of a novel disintegrin, saxatilin, in recombinant Pichia pastoris
Abstract

ABSTRACTThe methylotrophic yeast Pichia pastoris is one of the best hosts for the production of foreign proteins because of the presence of a strong alcohol oxidase 1 (AOX1) promoter that can be induced by methanol. Feeding the yeast, methanol induces protein production and provides an energy source for the host cells. However, excessive levels of methanol inhibit the growth of host cells, and insufficient methanol levels lead to poor growth and protein production. We have used various methanol feeding strategies to enhance the production of saxatilin. Saxatilin is a novel snake venom-derived disintegrin that inhibits tumor angiogenesis and metastasis and has been shown to suppress ovarian cancer cell invasion. A two-step increase feeding strategy to control the specific growth rate led to the best results in terms of specific protein production rates and final saxatilin amounts within the limited fermentation time.

Keywords
Methylotrophic yeast; Pichia pastoris; Saxatilin
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Control of specific growth rate to enhance the production of a novel disintegrin, saxatilin, in recombinant Pichia pastoris
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 3, September 2010, Pages 314–319
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us