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Development of a solid-state fermentation process for production of an alpha amylase with potentially interesting properties

Paper ID Volume ID Publish Year Pages File Format Full-Text
21101 43206 2010 5 PDF Available
Title
Development of a solid-state fermentation process for production of an alpha amylase with potentially interesting properties
Abstract

Ca-independency with potential activity and stability at low pH are among the most interesting characteristics of α-amylase in starch industry. In this attempt the synergetic effect of low pH on activity of crude Ca-independent α-amylase isolated from a native Bacillus sp. KR-8104 in solid-state fermentation (SSF) was studied using wheat bran (WB) as a substrate. The effects of different parameters including moisturizing agents, solid substrate to moisture ratio, particle size, incubation temperature and period, inoculum (v/w) and supplementation with 1% (w/w) different carbon and nitrogen sources on enzyme production were investigated. Maximum enzyme production of 140 U/g dry fermented substrate was obtained from wheat bran moistened with tap water at a ratio of 1:1.5 and supplemented with 1% (w/w) NH4NO3 and 1% (w/w) lactose after 48 h incubation at 37 °C. Even though the production of α-amylase was lower at 40 and 45 °C, the viable cell count was higher. In addition response surface methodology (RSM) was applied to find optimum conditions of temperature and pH on crude amylase activity. Using central composite design (CCD) a quadratic mathematical model equation was derived for the prediction of enzyme activity. The results showed that the model was in good agreement with experimental results, with R2 = 0.90 (p < 0.0001) and the low pH has a synergetic effect on enzyme activity at higher temperature.

Keywords
Bacillus sp.; Solid-state fermentation; Synergetic effect; Low pH; Temperature; Crude α-amylase activity; Response surface methodology
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Development of a solid-state fermentation process for production of an alpha amylase with potentially interesting properties
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 3, September 2010, Pages 333–337
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us