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Direct measurement of oxygen concentration inside cultured cartilage for relating to spatial growth of rabbit chondrocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
21106 43206 2010 4 PDF Available
Title
Direct measurement of oxygen concentration inside cultured cartilage for relating to spatial growth of rabbit chondrocytes
Abstract

In static culture of rabbit chondrocytes in collagen gel, the direct measurement of dissolved oxygen (DO) concentration revealed that the DO level at the top surface of gel decreased due to an increase in overall cell density with elapsed time. The local cell density at the top surface on day 21 was 5.7 × 107 cells/cm3, being 11 times that at the bottom of gel. This heterogeneity of cell distribution in the gel was considered to occur by limitation of oxygen supply into a deeper part of the gel. In shaking culture using a dish with gas-permeable film, the DO level was enhanced inside the gel and the overall cell density in the gel was achieved to be 2.9 times that in the static culture.

Keywords
Tissue engineering; Cultured cartilage; Chondrocytes; Dissolved oxygen measurement; Spatial cell distribution
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Direct measurement of oxygen concentration inside cultured cartilage for relating to spatial growth of rabbit chondrocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 3, September 2010, Pages 363–366
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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