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Enhancement of fibroblastic proliferation on chitosan surfaces by immobilized epidermal growth factor

Paper ID Volume ID Publish Year Pages File Format Full-Text
2111 100 2008 8 PDF Available
Title
Enhancement of fibroblastic proliferation on chitosan surfaces by immobilized epidermal growth factor
Abstract

Chitosan membranes were modified with mouse epidermal growth factor (EGF) by a photochemical technique. Photochemical immobilization was performed via a two-step process, in which EGF was first reacted with a heterobifunctional cross-linker sulfo-SANPAH (sulfosuccinimidyl 6(4′-azido-2′-nitrophenyl-amino)hexanoate) and then immobilized on the chitosan membrane by UV irradiation. The success of immobilization process was checked by Fourier transform infrared attenuated total reflection spectroscopy and X-ray photoelectron spectroscopy. Atomic force microscopy was used to evaluate the surface topography. The mitogenic effect of the EGF-modified chitosan membrane was investigated using mouse fibroblasts (L929 cell line), and cell proliferation was investigated by MTT and crystal violet assays. The results obtained from cell culture experiments showed that immobilized EGF stimulated fibroblast growth on chitosan membranes, and a considerable difference in cell proliferation was detected on EGF-modified chitosan membranes.

Keywords
Chitosan membrane; EGF; Photochemical technique; Cell proliferation; Mouse fibroblasts
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Enhancement of fibroblastic proliferation on chitosan surfaces by immobilized epidermal growth factor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 4, Issue 4, July 2008, Pages 989–996
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us