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Production of peritrichate bacterionanofibers and their proteinaceous components by Acinetobacter sp. Tol 5 cells affected by growth substrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
21118 43207 2011 6 PDF Available
Title
Production of peritrichate bacterionanofibers and their proteinaceous components by Acinetobacter sp. Tol 5 cells affected by growth substrates
Abstract

The toluene-degrading bacterium Acinetobacter sp. Tol 5 is highly adhesive through cell-surface nanofibers. Previously, we identified two morphologically distinct nanofibers on Tol 5 cells, namely, nonperitrichate anchor-like and peritrichate pilus-like nanofibers. In the present study, the application of improved electron microscopy techniques enabled discrimination of three distinct types of peritrichate nanofibers on Tol 5 cells. Interestingly, production of these nanofibers was affected by the available growth substrate. Thick, long, straight nanofibers a, which were present on cells grown on toluene, lactate, and ethanol, were not observed on cells grown on triacylglycerol (TAG). In contrast, cells grown on TAG were covered with long, curved nanofibers c, which only existed sparsely on cells grown on toluene, lactate, and ethanol. Thin, short, straight nanofibers b were found densely covering the margin of cells grown on all four growth substrates. SDS-PAGE of Tol 5 cell-surface proteins detected a protein of 17.5 kDa that was expressed at a high level on ethanol, but was undetectable on TAG. Conversely, a 26 kDa protein was identified that was exclusively expressed on TAG, but was only faintly expressed by cells grown on the other substrates. Based on N-terminal amino acid sequences, the 17.5 and 26 kDa proteins were identified as the major subunits of type 1 and Fil pili, respectively, which are typical bacterionanofibers. From these results, we deduced that nanofibers a and c are type 1 and Fil pili, respectively. The adhesiveness of Tol 5 cells was low only when they were grown on TAG.

Keywords
Acinetobacter; Bacterionanofibers; Carbon source; Pili; Adhesion
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Production of peritrichate bacterionanofibers and their proteinaceous components by Acinetobacter sp. Tol 5 cells affected by growth substrates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 1, January 2011, Pages 31–36
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us