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Widely targeted metabolic profiling analysis of yeast central metabolites

Paper ID Volume ID Publish Year Pages File Format Full-Text
21153 43208 2012 9 PDF Available
Title
Widely targeted metabolic profiling analysis of yeast central metabolites
Abstract

A method for a widely targeted analysis was developed for the metabolic profiling of yeast central metabolism. The widely targeted method consists of 2 analyses, namely, gas chromatography–quadrupole–mass spectrometry (GC–Q–MS) operated in selected ion monitoring mode with 25 m/z channels, and liquid chromatography triple–stage quadrupole (LC–QqQ)–MS operated in multiple reaction monitoring mode. This platform was set up to identify and quantify preselected 99 compounds, including sugars, sugar phosphates, organic acids, amino acids, and cofactors. The method showed good sensitivity and a wide dynamic range. For example, limits of detection for lactate and l-phenylalanine were 1.4 fmol and 2.0 fmol, respectively. The dynamic ranges for GC–Q–MS analysis and LC–QqQ–MS analysis were approximately 102–105 and 103–104, respectively. The metabolite profiles of 2 yeast strains, YPH499 and BY4741, under glucose-fermenting conditions were compared using the developed method. Although YPH499 and BY4741 were derived from an identical experimental strain, the profiling analysis successfully revealed a variation in metabolic phenotypes among experimental yeast strains demonstrating that the widely targeted method could be a robust and useful method for the investigation of metabolic phenotypes of Saccharomyces cerevisiae.

Keywords
Saccharomyces cerevisiae; Widely targeted metabolic profiling analysis; Central metabolism; Quadrupole mass spectrometer; Gas chromatography; Liquid chromatography
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 5, May 2012, Pages 665–673
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us